Our new Distinct+ tissue clearing strategy is the one process that delipidates samples with no alter in morphology and with small influence on structural integrity.
Megatome is really a vibrating microtome created to area a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables higher-throughput tissue sectioning with uniform surface area profile, and also small tissue injury and data reduction.
Antibodies may perhaps choose weeks to diffuse by way of just a few millimeters of tissue, by using a steep labeling gradient from floor to core.
Megatome is made for accuracy: the blade vibrates at the next frequency and larger amplitude assortment than other microtomes, and includes a special deflection Handle mechanism.
SE makes use of a rotational electric field to disperse highly electromobile molecules (which include antibodies or surfactant micelles) all over a porous sample devoid of detrimental electrically charged structures within the tissue. This allows two-4 day clearing of intact organs,
Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them absolutely clear. This permits light penetration into your sample and makes certain the acquisition of substantial-resolution, in-concentrate impression info.
Megatome is actually a novel microtome which allows for prime-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human Mind hemispheres – with minimum tissue destruction and data loss.
It’s time to up grade your microtome to Megatome. With correct high-frequency slicing for an unmatched choice of sample sizes and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for numerous purposes.
The SmartSPIM mild sheet microscope presents unparalleled resolution and acquisition pace all over intact tissue volumes, although its modular structure lets you update and customize particular person components.
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Defend avoids the variability of hydrogel embedding and the data loss from PFA preservation, megatomi.com safeguarding specimens for various rounds of processing.
Leverage the Very clear+ tissue clearing system, together with eFLASH and patented stochastic electrotransport technologies, to rapidly crystal clear and label full organs. Important highlights and functions incorporate:
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